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Journal of Biomedical Optics

Vertically scanned laser sheet microscopy
Author(s): Di Dong; Alicia Arranz; Shouping Zhu; Yujie Yang; Liangliang Shi; Jun Wang; Chen Shen; Jie Tian; Jorge Ripoll
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Paper Abstract

Laser sheet microscopy is a widely used imaging technique for imaging the three-dimensional distribution of a fluorescence signal in fixed tissue or small organisms. In laser sheet microscopy, the stripe artifacts caused by high absorption or high scattering structures are very common, greatly affecting image quality. To solve this problem, we report here a two-step procedure which consists of continuously acquiring laser sheet images while vertically displacing the sample, and then using the variational stationary noise remover (VSNR) method to further reduce the remaining stripes. Images from a cleared murine colon acquired with a vertical scan are compared with common stitching procedures demonstrating that vertically scanned light sheet microscopy greatly improves the performance of current light sheet microscopy approaches without the need for complex changes to the imaging setup and allows imaging of elongated samples, extending the field of view in the vertical direction.

Paper Details

Date Published: 1 October 2014
PDF: 8 pages
J. Biomed. Opt. 19(10) 106001 doi: 10.1117/1.JBO.19.10.106001
Published in: Journal of Biomedical Optics Volume 19, Issue 10
Show Author Affiliations
Di Dong, Institute of Automation (China)
Alicia Arranz, ETH Zürich (Switzerland)
Shouping Zhu, Xidian Univ. (China)
Yujie Yang, Institute of Automation (China)
Liangliang Shi, Institute of Automation (China)
Jun Wang, Harbin Univ. of Science and Technology (China)
Chen Shen, Xidian Univ. (China)
Jie Tian, Institute of Automation (China)
Jorge Ripoll, Univ. Carlos III de Madrid (Spain)
Instituto de Investigación Sanitaria del Hospital Gregorio Marañón (Spain)
Foundation for Research and Technology-Hellas (Greece)


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