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Journal of Biomedical Optics

Rapid, low-cost fluorescent assay of β-lactamase-derived antibiotic resistance and related antibiotic susceptibility
Author(s): Sultan Sibel Erdem; Shazia Khan; Akilan Palanisami; Tayyaba Hasan
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Paper Abstract

Antibiotic resistance (AR) is increasingly prevalent in low and middle income countries (LMICs), but the extent of the problem is poorly understood. This lack of knowledge is a critical deficiency, leaving local health authorities essentially blind to AR outbreaks and crippling their ability to provide effective treatment guidelines. The crux of the problem is the lack of microbiology laboratory capacity available in LMICs. To address this unmet need, we demonstrate a rapid and simple test of β-lactamase resistance (the most common form of AR) that uses a modified β-lactam structure decorated with two fluorophores quenched due to their close proximity. When the β-lactam core is cleaved by β-lactamase, the fluorophores dequench, allowing assay speeds of 20 min to be obtained with a simple, streamlined protocol. Furthermore, by testing in competition with antibiotics, the β-lactamase-associated antibiotic susceptibility can also be extracted. This assay can be easily implemented into standard lab work flows to provide near real-time information of β-lactamase resistance, both for epidemiological purposes as well as individualized patient care.

Paper Details

Date Published: 10 October 2014
PDF: 11 pages
J. Biomed. Opt. 19(10) 105007 doi: 10.1117/1.JBO.19.10.105007
Published in: Journal of Biomedical Optics Volume 19, Issue 10
Show Author Affiliations
Sultan Sibel Erdem, Wellman Ctr. for Photomedicine (United States)
Shazia Khan, Wellman Ctr. for Photomedicine (United States)
Akilan Palanisami, Wellman Ctr. for Photomedicine (United States)
Tayyaba Hasan, Wellman Ctr. for Photomedicine (United States)

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