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Journal of Biomedical Optics • Open Access

Cellular level nanomanipulation using atomic force microscope aided with superresolution imaging
Author(s): Jenu Varghese Chacko; Benjamin Harke; Claudio Canale; Alberto Diaspro

Paper Abstract

Atomic force microscopes (AFM) provide topographical and mechanical information of the sample with very good axial resolution, but are limited in terms of chemical specificity and operation time-scale. An optical microscope coupled to an AFM can recognize and target an area of interest using specific identification markers like fluorescence tags. A high resolution fluorescence microscope can visualize fluorescence structures or molecules below the classical optical diffraction limit and reach nanometer scale resolution. A stimulated emission depletion (STED) microscopy superresolution (SR) microscope coupled to an AFM is an example in which the AFM tip gains nanoscale manipulation capabilities. The SR targeting and visualization ability help in fast and specific identification of subdiffraction-sized cellular structures and manoeuvring the AFM tip onto the target. We demonstrate how to build a STED AFM and use it for biological nanomanipulation aided with fast visualization. The STED AFM based bionanomanipulation is presented for the first time in this article. This study points to future nanosurgeries performable at single-cell level and a physical targeted manipulation of cellular features as it is currently used in research domains like nanomedicine and nanorobotics.

Paper Details

Date Published: 7 October 2014
PDF: 9 pages
J. Biomed. Opt. 19(10) 105003 doi: 10.1117/1.JBO.19.10.105003
Published in: Journal of Biomedical Optics Volume 19, Issue 10
Show Author Affiliations
Jenu Varghese Chacko, Istituto Italiano di Tecnologia (Italy)
Univ. degli Studi di Genova (Italy)
Benjamin Harke, Istituto Italiano di Tecnologia (Italy)
Abberior Instruments GmbH (Germany)
Claudio Canale, Istituto Italiano di Tecnologia (Italy)
Alberto Diaspro, Istituto Italiano di Tecnologia (Italy)
Univ. degli Studi di Genova (Italy)
Nikon Imaging Center at Istituto Italiano di Tecnologia (Italy)


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