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Journal of Biomedical Optics

980-nm infrared laser modulation of sodium channel kinetics in a neuron cell linearly mediated by photothermal effect
Author(s): Xinyu Li; Jia Liu; Shanshan Liang; Changsen Sun
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Paper Abstract

Photothermal effect (PE) plays a major role in the near-infrared laser interaction with biological tissue. But, quite few interactions can be quantitatively depicted. Here, a two-step model is proposed to describe a 980-nm infrared laser interaction with neuron cell in vitro. First, the laser-induced temperature rises in the cell surrounding area were measured by using an open pipette method and also calculated by solving the heat conduction equation. Second, we recorded the modifications on sodium (Na) channel current in neuron cells directly by using a patch clamp to synchronize the 980-nm laser irradiation and obtained how the electrophysiological function of neuron cells respond to the temperature rise. Then, the activation time constants, τm, were extracted by fitting the sodium currents with the Hodgkin–Huxley model. The infrared laser modulation effect on sodium currents kinetics was examined by taking a ratio between the time constants with and without the laser irradiations. The analysis revealed that the averaged ratio at a specific laser exposure could be well related to the temperature properties of the Na channel protein. These results proved that the modulation of sodium current kinetics of a neuron cell in vitro by 980-nm laser with different-irradiation levels was linearly mediated corresponding to the laser-induced PE.

Paper Details

Date Published: 7 October 2014
PDF: 6 pages
J. Biomed. Opt. 19(10) 105002 doi: 10.1117/1.JBO.19.10.105002
Published in: Journal of Biomedical Optics Volume 19, Issue 10
Show Author Affiliations
Xinyu Li, Dalian Univ. of Technology (China)
Jia Liu, Dalian Univ. of Technology (China)
Shanshan Liang, Dalian Univ. of Technology (China)
Changsen Sun, Dalian Univ. of Technology (China)

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