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Journal of Biomedical Optics

Differential sensitivity of osteoblasts and bacterial pathogens to 405-nm light highlighting potential for decontamination applications in orthopedic surgery
Author(s): Praveen Ramakrishnan; Michelle Maclean; Scott J. MacGregor; John G. Anderson; M. Helen Grant
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Paper Abstract

Healthcare associated infections pose a major threat to patients admitted to hospitals and infection rates following orthopedic arthroplasty surgery are as high as 4%. A 405-nm high-intensity narrow spectrum light has been proven to reduce environmental contamination in hospital isolation rooms, and there is potential to develop this technology for application in arthroplasty surgery. Cultured rat osteoblasts were exposed to varying light intensities and it was found that exposures of up to a dose of 36  J/cm2 had no significant effect on cell viability [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay], function (alkaline phosphatase activity), and proliferation rate (BrdU cell proliferation assay). High irradiance exposures (54  J/cm2) significantly affected the cell viability indicating that the effects of 405-nm light on osteoblasts are dose dependent. Additionally, exposure of a variety of clinically related bacteria to a dose of 36  J/cm2 resulted in up to 100% kill. These results demonstrating the differential sensitivity of osteoblasts and bacteria to 405-nm light are an essential step toward developing the technique for decontamination in orthopedic surgery.

Paper Details

Date Published: 2 October 2014
PDF: 7 pages
J. Biomed. Opt. 19(10) 105001 doi: 10.1117/1.JBO.19.10.105001
Published in: Journal of Biomedical Optics Volume 19, Issue 10
Show Author Affiliations
Praveen Ramakrishnan, Univ. of Strathclyde (United Kingdom)
Michelle Maclean, Univ. of Strathclyde (United Kingdom)
Scott J. MacGregor, Univ. of Strathclyde (United Kingdom)
John G. Anderson, Univ. of Strathclyde (United Kingdom)
M. Helen Grant, Univ. of Strathclyde (United Kingdom)

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