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Journal of Biomedical Optics • Open Access

Noninvasive near-infrared fluorescent protein-based imaging of tumor progression and metastases in deep organs and intraosseous tissues
Author(s): Carine Jiguet-Jiglaire; Mylene Cayol; Sylvie Mathieu; Charlotte Jeanneau; Corinne Bouvier-Labit; L'houcine Ouafik; Assou El-Battari

Paper Abstract

Whole-body imaging of experimental tumor growth is more feasible within the near-infrared (NIR) optical window because of the highest transparency of mammalian tissues within this wavelength spectrum, mainly due to improved tissue penetration and lower autofluorescence. We took advantage from the recently cloned infrared fluorescent protein (iRFP) together with a human immunodeficiency virus (HIV)-based lentiviral vector to produce virally transduced tumor cells that permanently express this protein. We then noninvasively explored metastatic spread as well as primary tumor growth in deep organs and behind bone barriers. Intrabone tumor growth was investigated through intracranial and intratibial injections of glioblastoma and osteosarcoma cells, respectively, and metastasis was assessed by tail vein injection of melanoma cells. We found that the emitted fluorescence is captured as sharp images regardless of the organ or tissue considered. Furthermore, by overlaying fluorescence spots with the white light, it was possible to afford whole-body images yet never observed before. This approach allowed us to continuously monitor the growth and dissemination of tumor cells with a small number of animals, minimal animal handling, and without the need for any additive. This iRFP-based system provides high-resolution readouts of tumorigenesis that should greatly facilitate preclinical trials with anticancer therapeutic molecules.

Paper Details

Date Published: 27 January 2014
PDF: 7 pages
J. Biomed. Opt. 19(1) 016019 doi: 10.1117/1.JBO.19.1.016019
Published in: Journal of Biomedical Optics Volume 19, Issue 1
Show Author Affiliations
Carine Jiguet-Jiglaire, Institut National de la Santé et de la Recherche Médicale (France)
Mylene Cayol, Institut National de la Santé et de la Recherche Médicale (France)
Sylvie Mathieu, Institut National de la Santé et de la Recherche Médicale (France)
Charlotte Jeanneau, Institut National de la Santé et de la Recherche Médicale (France)
Corinne Bouvier-Labit, Institut National de la Santé et de la Recherche Médicale (France)
L'houcine Ouafik, Institut National de la Santé et de la Recherche Médicale (France)
Assou El-Battari, Institut National de la Santé et de la Recherche Médicale (France)


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