Share Email Print
cover

Journal of Biomedical Optics • Open Access

Spatial frequency analysis of anisotropic drug transport in tumor samples

Paper Abstract

Directional Fourier spatial frequency analysis was used on standard histological sections to identify salient directional bias in the spatial frequencies of stromal and epithelial patterns within tumor tissue. This directional bias is shown to be correlated to the pathway of reduced fluorescent tracer transport. Optical images of tumor specimens contain a complex distribution of randomly oriented aperiodic features used for neoplastic grading that varies with tumor type, size, and morphology. The internal organization of these patterns in frequency space is shown to provide a precise fingerprint of the extracellular matrix complexity, which is well known to be related to the movement of drugs and nanoparticles into the parenchyma, thereby identifying the characteristic spatial frequencies of regions that inhibit drug transport. The innovative computational methodology and tissue validation techniques presented here provide a tool for future investigation of drug and particle transport in tumor tissues, and could potentially be used a priori to identify barriers to transport, and to analyze real-time monitoring of transport with respect to therapeutic intervention.

Paper Details

Date Published: 6 January 2014
PDF: 7 pages
J. Biomed. Opt. 19(1) 015005 doi: 10.1117/1.JBO.19.1.015005
Published in: Journal of Biomedical Optics Volume 19, Issue 1
Show Author Affiliations
Stewart Russell, Thayer School of Engineering at Dartmouth (United States)
Kimberley S. Samkoe, Geisel School of Medicine (United States)
Jason R. Gunn, Thayer School of Engineering at Dartmouth (United States)
P. Jack Hoopes, Geisel School of Medicine (United States)
Thienan A. Nguyen, The City College of New York (United States)
Milo J. Russell, The City College of New York (United States)
Robert R. Alfano, The City College of New York (United States)
Brian W. Pogue, Thayer School of Engineering at Dartmouth (United States)


© SPIE. Terms of Use
Back to Top