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Journal of Biomedical Optics

Rapid detection of urinary polyomavirus BK by heterodyne-based surface plasmon resonance biosensor
Author(s): Li-Chen Su; Ya-Chung Tian; Ying-Feng Chang; Chien Chou; Chao-Sung Lai
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Paper Abstract

In renal transplant patients, immunosuppressive therapy may result in the reactivation of polyomavirus BK (BKV), leading to polyomavirus-associated nephropathy (PVAN), which inevitably causes allograft failure. Since the treatment outcomes of PVAN remain unsatisfactory, early identification and continuous monitoring of BKV reactivation and reduction of immunosuppressants are essential to prevent PVAN development. The present study demonstrated that the developed dual-channel heterodyne-based surface plasmon resonance (SPR) biosensor is applicable for the rapid detection of urinary BKV. The use of a symmetrical reference channel integrated with the poly(ethylene glycol)-based low-fouling self-assembled monolayer to reduce the environmental variations and the nonspecific noise was proven to enhance the sensitivity in urinary BKV detection. Experimentally, the detection limit of the biosensor for BKV detection was estimated to be around 8500  copies/mL . In addition, urine samples from five renal transplant patients were tested to rapidly distinguish PVAN-positive and PVAN-negative renal transplant patients. By virtue of its simplicity, rapidity, and applicability, the SPR biosensor is a remarkable potential to be used for continuous clinical monitoring of BKV reactivation.

Paper Details

Date Published: 20 September 2013
PDF: 7 pages
J. Biomed. Opt. 19(1) 011013 doi: 10.1117/1.JBO.19.1.011013
Published in: Journal of Biomedical Optics Volume 19, Issue 1
Show Author Affiliations
Li-Chen Su, Chang Gung Univ. (Taiwan)
Ya-Chung Tian, Chang-Gung Memorial Hospital (Taiwan)
Ying-Feng Chang, Chang Gung Univ. (Taiwan)
Chien Chou, Chang Gung Univ. (Taiwan)
Chao-Sung Lai, Chang Gung Univ. (Taiwan)


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