Share Email Print

Journal of Biomedical Optics

Lipid droplet pattern and nondroplet-like structure in two fat/i] mutants of Caenorhabditis elegans revealed by coherent anti-Stokes Raman scattering microscopy
Author(s): Yung-Hsiang Yi; Cheng-Hao Chien; Wei-Wen Chen; Tian-Hsiang Ma; Kuan-Yu Liu; Yu-Sun Chang; Ta-Chau Chang; Szecheng J. Lo
Format Member Price Non-Member Price
PDF $20.00 $25.00

Paper Abstract

Lipid is an important energy source and essential component for plasma and organelle membranes in all kinds of cells. Coherent anti-Stokes Raman scattering (CARS) microscopy is a label-free and nonlinear optical technique that can be used to monitor the lipid distribution in live organisms. Here, we utilize CARS microscopy to investigate the pattern of lipid droplets in two live Caenorhabditis elegans mutants (fat-2 and fat-3). The CARS images showed a striking decrease in the size, number, and content of lipid droplets in the fat-2 mutant but a slight difference in the fat-3 mutant as compared with the wild-type worm. Moreover, a nondroplet-like structure with enhanced CARS signal was detected for the first time in the uterus of fat-2 and fat-3 mutants. In addition, transgenic fat-2 mutant expressing a GFP fusion protein of vitellogenin-2 (a yolk lipoprotein) revealed that the enhanced CARS signal colocalized with the GFP signal, which suggests that the nondroplet-like structure is primarily due to the accumulation of yolk lipoproteins. Together, this study implies that CARS microscopy is a potential tool to study the distribution of yolk lipoproteins, in addition to lipid droplets, in live animals.

Paper Details

Date Published: 26 August 2013
PDF: 7 pages
J. Biomed. Opt. 19(1) 011011 doi: 10.1117/1.JBO.19.1.011011
Published in: Journal of Biomedical Optics Volume 19, Issue 1
Show Author Affiliations
Yung-Hsiang Yi, Chang Gung Univ. (Taiwan)
Cheng-Hao Chien, Academia Sinica (Taiwan)
Wei-Wen Chen, Academia Sinica (Taiwan)
Tian-Hsiang Ma, Chang Gung Univ. (Taiwan)
Kuan-Yu Liu, Chang Gung Univ. (Taiwan)
Yu-Sun Chang, Chang Gung Univ. (Taiwan)
Ta-Chau Chang, Academia Sinica (Taiwan)
Szecheng J. Lo, Chang Gung Univ. (Taiwan)

© SPIE. Terms of Use
Back to Top