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Journal of Biomedical Optics • Open Access

Using optical profilometry to characterize cell membrane roughness influenced by amyloid-beta 42 aggregates and electric fields
Author(s): Huei-Jyuan Pan; Ruei-Lin Wang; Jian-Long Xiao; Yu-Jen Chang; Ji-Yen Cheng; Yu-Jen Chang; Chau-Hwang Lee

Paper Abstract

The membrane roughness of Neuro-2a neroblastoma cells is measured by using noninterferometric wide-field optical profilometry. The cells are treated with the fibril and oligomer conformers of amyloid-beta (Aβ ) 42, which is a peptide of 42 amino acids related to the development of Alzheimer’s disease. We find that both the Aβ42 fibrils and Aβ42 oligomers reduced the cell membrane roughness, but the effect of Aβ42 oligomers was faster and stronger than that of the fibrils. We also apply direct-current electric field (dcEF) stimulations on the cells. A dcEF of 300  mV/mm can increase the membrane roughness under the treatment of Aβ42 . These results suggest that Aβ42 can decrease the membrane compliance of live neuroblastoma cells, and dcEFs may counteract this effect.

Paper Details

Date Published: 26 July 2013
PDF: 5 pages
J. Biomed. Opt. 19(1) 011009 doi: 10.1117/1.JBO.19.1.011009
Published in: Journal of Biomedical Optics Volume 19, Issue 1
Show Author Affiliations
Huei-Jyuan Pan, Research Ctr. for Applied Sciences (Taiwan)
Ruei-Lin Wang, National Yang-Ming Univ. (Taiwan)
Jian-Long Xiao, Research Ctr. for Applied Sciences (Taiwan)
Yu-Jen Chang, Research Ctr. for Applied Sciences (Taiwan)
Ji-Yen Cheng, Research Ctr. for Applied Sciences (Taiwan)
Yu-Jen Chang, Research Ctr. for Applied Sciences (Taiwan)
Chau-Hwang Lee, Research Ctr. for Applied Sciences (Taiwan)


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