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Journal of Biomedical Optics • Open Access

Determination of urine cofilin-1 level in acute kidney injury using a high-throughput localized surface plasmon-coupled fluorescence biosensor
Author(s): Ying-Feng Chang; Cheng-Han Chao; Lih-Yuan Lin; Cheng-Han Tsai; Chien Chou; Yi-Jang Lee

Paper Abstract

The actin-depolymerizing factor (ADF)/cofilin protein family has been reported to be associated with ischemia-induced renal disorders. We examine whether cofilin-1 is associated with acute kidney injury (AKI) using human urine samples. We exploited a 96-well based high-throughput biosensor that uses gold nanoparticles and a sandwich immunoassay to detect the urine cofilin-1 level of AKI patients. The mean urine cofilin-1 level of the AKI patients (n=37 from 47 cases analyzed) was twofold higher than that of healthy adults (n=21 from 29 cases analyzed). The receiver operating characteristic (ROC) curve showed that cofilin-1 was acceptable for discriminating AKI patients from healthy adults. However, an increase of the sample size is required to conclude the importance of urine cofilin-1 on AKI diagnosis, and the high-throughput ultrasensitive biosensor used in this study would greatly accelerate the measurement of urine cofilin-1 in an increased sample size.

Paper Details

Date Published: 20 June 2013
PDF: 7 pages
J. Biomed. Opt. 19(1) 011004 doi: 10.1117/1.JBO.19.1.011004
Published in: Journal of Biomedical Optics Volume 19, Issue 1
Show Author Affiliations
Ying-Feng Chang, Chang Chung Univ. (Taiwan)
Cheng-Han Chao, National Taiwan Univ. Hospital, Hsin-Chu Branch (Taiwan)
Lih-Yuan Lin, National Tsing Hua Univ. (Taiwan)
Cheng-Han Tsai, National Yang-Ming Univ. (Taiwan)
Chien Chou, Chang Gung Univ. (Taiwan)
Yi-Jang Lee, National Yang-Ming Univ. (Taiwan)

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