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Journal of Biomedical Optics

Observation of the dynamic germination of single bacterial spores using rapid Raman imaging
Author(s): Lingbo Kong; Peter Setlow; Yong-qing Li
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Paper Abstract

The dynamics of bacterial spore germination were successfully observed using a fast Raman imaging system, in combination with real-time phase contrast microscopy. By using a multifocus scan scheme, the spontaneous Raman-scattering imaging acquisition speed was increased to ∼30  s per frame while maintaining diffraction-limited resolution, which allowed monitoring of the dynamics of spore germination on a time scale of tens of seconds to a few minutes. This allowed simultaneous gathering of rich spatial distribution information on different cellular components including time-lapse molecular images of Ca-dipicolinic acid, protein, and nucleic acid during germination of single bacterial spores for the periods of 30 to 60 min.

Paper Details

Date Published: 10 July 2013
PDF: 7 pages
J. Biomed. Opt. 19(1) 011003 doi: 10.1117/1.JBO.19.1.011003
Published in: Journal of Biomedical Optics Volume 19, Issue 1
Show Author Affiliations
Lingbo Kong, East Carolina Univ. (United States)
Peter Setlow, Univ. of Connecticut Health Ctr. (United States)
Yong-qing Li, East Carolina Univ. (United States)


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