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Journal of Biomedical Optics • Open Access

Ultrastructural alterations in field carcinogenesis measured by enhanced backscattering spectroscopy
Author(s): Andrew J. Radosevich; Nikhil N. Mutyal; Ji Yi; Yolanda Stypula-Cyrus; Jeremy D. Rogers; Michael J. Goldberg; Laura Bianchi; Shailesh Bajaj; Hemant K. Roy

Paper Abstract

Optical characterization of biological tissue in field carcinogenesis offers a method with which to study the mechanisms behind early cancer development and the potential to perform clinical diagnosis. Previously, low-coherence enhanced backscattering spectroscopy (LEBS) has demonstrated the ability to discriminate between normal and diseased organs based on measurements of histologically normal-appearing tissue in the field of colorectal (CRC) and pancreatic (PC) cancers. Here, we implement the more comprehensive enhanced backscattering (EBS) spectroscopy to better understand the structural and optical changes which lead to the previous findings. EBS provides high-resolution measurement of the spatial reflectance profile P(r s ) between 30 microns and 2.7 mm, where information about nanoscale mass density fluctuations in the mucosa can be quantified. A demonstration of the length-scales at which P(r s ) is optimally altered in CRC and PC field carcinogenesis is given and subsequently these changes are related to the tissue’s structural composition. Three main conclusions are made. First, the most significant changes in P(r s ) occur at short length-scales corresponding to the superficial mucosal layer. Second, these changes are predominantly attributable to a reduction in the presence of subdiffractional structures. Third, similar trends are seen for both cancer types, suggesting a common progression of structural alterations in each.

Paper Details

Date Published: 5 September 2013
PDF: 12 pages
J. Biomed. Opt. 18(9) 097002 doi: 10.1117/1.JBO.18.9.097002
Published in: Journal of Biomedical Optics Volume 18, Issue 9
Show Author Affiliations
Andrew J. Radosevich, Northwestern Univ. (United States)
Nikhil N. Mutyal, Northwestern Univ. (United States)
Ji Yi, Northwestern Univ. (United States)
Yolanda Stypula-Cyrus, Northwestern Univ. (United States)
Jeremy D. Rogers, Northwestern Univ. (United States)
Michael J. Goldberg, NorthShore Univ. HealthSystem (United States)
Laura Bianchi, NorthShore Univ. HealthSystem (United States)
Shailesh Bajaj, NorthShore Univ. HealthSystem (United States)
Hemant K. Roy, Boston Medical Ctr. (United States)

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