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Journal of Biomedical Optics

Investigation of transport dynamics in oleic acid–induced transdermal drug delivery by two-photon fluorescence microscopy: an ex-vivo study of mouse skin
Author(s): Te-Yu Tseng; Hsun-Chia Hsu; Chiu-Sheng Yang; Tsung-Hua Tsai; Chen-Yuan Dong
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Paper Abstract

Transdermal drug delivery, transporting the drug molecules through epidermis to dermis, has been extensively investigated but not studied in dynamic detail. The objective of this study is to monitor the dynamical changes of drug permeation and that of polarization of skin tissue with oleic acid treatment. We utilize two-photon fluorescence microscopy (TPM) to investigate the dynamics of transdermal drug delivery in skin excised from the abdominal region of euthenized nude mice with sulforhodamine B (SRB) modeling as a drug and Laurdan serving as a polarity indicator. The treatment of oleic acid increases the permeation rate of SRB, quickly reaching to the steady state of permeation. Increases in polarity within the skin tissue (in both intercellular and intracellular region of stratum corneum) are observed in SRB permeation enhanced by oleic acid treatment. TPM has successfully demonstrated the ability to study spatial distribution of transport dynamics in oleic acid-enhanced transdermal delivery.

Paper Details

Date Published: 25 September 2013
PDF: 8 pages
J. Biomed. Opt. 18(9) 096016 doi: 10.1117/1.JBO.18.9.096016
Published in: Journal of Biomedical Optics Volume 18, Issue 9
Show Author Affiliations
Te-Yu Tseng, National Taiwan Univ. (Taiwan)
Hsun-Chia Hsu, National Taiwan Univ. (Taiwan)
Chiu-Sheng Yang, National Taiwan Univ. (Taiwan)
Tsung-Hua Tsai, Far Eastern Memorial Hospital (Taiwan)
Chen-Yuan Dong, National Taiwan Univ. (Taiwan)


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