Share Email Print
cover

Journal of Biomedical Optics • Open Access

Visible spatial frequency domain imaging with a digital light microprojector

Paper Abstract

There is a need for cost effective, quantitative tissue spectroscopy and imaging systems in clinical diagnostics and pre-clinical biomedical research. A platform that utilizes a commercially available light-emitting diode (LED) based projector, cameras, and scaled Monte Carlo model for calculating tissue optical properties is presented. These components are put together to perform spatial frequency domain imaging (SFDI), a model-based reflectance technique that measures and maps absorption coefficients (μ a ) and reduced scattering coefficients (μ ′ s ) in thick tissue such as skin or brain. We validate the performance of the flexible LED and modulation element (FLaME) system at 460, 530, and 632 nm across a range of physiologically relevant μ a values (0.07 to 1.5  mm −1 ) in tissue-simulating intralipid phantoms, showing an overall accuracy within 11% of spectrophotometer values for μ a and 3% for μ ′ s . Comparison of oxy- and total hemoglobin fits between the FLaME system and a spectrophotometer (450 to 1000 nm) is differed by 3%. Finally, we acquire optical property maps of a mouse brain in vivo with and without an overlying saline well. These results demonstrate the potential of FLaME to perform tissue optical property mapping in visible spectral regions and highlight how the optical clearing effect of saline is correlated to a decrease in μ ′ s of the skull.

Paper Details

Date Published: 4 September 2013
PDF: 8 pages
J. Biomed. Opt. 18(9) 096007 doi: 10.1117/1.JBO.18.9.096007
Published in: Journal of Biomedical Optics Volume 18, Issue 9
Show Author Affiliations
Alexander J. Lin, Beckman Laser Institute and Medical Clinic (United States)
Adrien Ponticorvo, Beckman Laser Institute and Medical Clinic (United States)
Soren D. Konecky, Beckman Laser Institute and Medical Clinic (United States)
Haotian Cui, Univ. of California, Irvine (United States)
Tyler B. Rice, Beckman Laser Institute and Medical Clinic (United States)
Bernard Choi, Beckman Laser Institute and Medical Clinic (United States)
Anthony J. Durkin, Beckman Laser Institute and Medical Clinic (United States)
Bruce J. Tromberg, Beckman Laser Institute and Medical Clinic (United States)


© SPIE. Terms of Use
Back to Top