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Journal of Biomedical Optics

Cross-correlation-based transverse flow measurements using optical resolution photoacoustic microscopy with a digital micromirror device
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Paper Abstract

A cross-correlation-based method is proposed to quantitatively measure transverse flow velocity using optical resolution photoacoustic (PA) microscopy enhanced with a digital micromirror device (DMD). The DMD is used to alternately deliver two spatially separated laser beams to the target. Through cross-correlation between the slow-time PA profiles measured from the two beams, the speed and direction of transverse flow are simultaneously derived from the magnitude and sign of the time shift, respectively. Transverse flows in the range of 0.50 to 6.84  mm/s are accurately measured using an aqueous suspension of 10-μm-diameter microspheres, and the root-mean-squared measurement accuracy is quantified to be 0.22  mm/s . The flow measurements are independent of the particle size for flows in the velocity range of 0.55 to 6.49  mm/s , which was demonstrated experimentally using three different sizes of microspheres (diameters: 3, 6, and 10 μm). The measured flow velocity follows an expected parabolic distribution along the depth direction perpendicular to the flow. Both maximum and minimum measurable velocities are investigated for varied distances between the two beams and varied total time for one measurement. This technique shows an accuracy of 0.35  mm/s at 0.3-mm depth in scattering chicken breast, making it promising for measuring flow in biological tissue.

Paper Details

Date Published: 3 September 2013
PDF: 8 pages
J. Biomed. Opt. 18(9) 096004 doi: 10.1117/1.JBO.18.9.096004
Published in: Journal of Biomedical Optics Volume 18, Issue 9
Show Author Affiliations
Jinyang Liang, Washington Univ. in St. Louis (United States)
Yong Zhou, Washington Univ. in St. Louis (United States)
Konstantin I. Maslov, Washington Univ. in St. Louis (United States)
Lihong V. Wang, Washington Univ. in St. Louis (United States)

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