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Journal of Biomedical Optics

Needle-based fluorescence endomicroscopy via structured illumination with a plastic, achromatic objective
Author(s): Matthew R. Kyrish; Jessica L. Dobbs; Shalini Jain; Xiao Wang; Dihua Yu; Rebecca R. Richards-Kortum; Tomasz S. Tkaczyk
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Paper Abstract

In order to diagnose cancer, a sample must be removed, prepared, and examined under a microscope, which is expensive, invasive, and time consuming. Fiber optic fluorescence endomicroscopy, where an image guide is used to obtain high-resolution images of tissue in vivo, has shown promise as an alternative to conventional biopsies. However, the resolution of standard endomicroscopy is limited by the fiber bundle sampling frequency and out-of-focus light. A system is presented which incorporates a plastic, achromatic objective to increase the sampling and which provides optical sectioning via structured illumination to reject background light. An image is relayed from the sample by a fiber bundle with the custom 2.1-mm outer diameter objective lens integrated to the distal tip. The objective is corrected for the excitation and the emission wavelengths of proflavine (452 and 515 nm). It magnifies the object onto the fiber bundle to improve the system’s lateral resolution by increasing the sampling. The plastic lenses were fabricated via single-point diamond turning and assembled using a zero alignment technique. Ex vivo images of normal and neoplastic murine mammary tissues stained with proflavine are captured. The system achieves higher contrast and resolves smaller features than standard fluorescence endomicroscopy.

Paper Details

Date Published: 3 September 2013
PDF: 8 pages
J. Biomed. Opt. 18(9) 096003 doi: 10.1117/1.JBO.18.9.096003
Published in: Journal of Biomedical Optics Volume 18, Issue 9
Show Author Affiliations
Matthew R. Kyrish, Rice Univ. (United States)
Jessica L. Dobbs, Rice Univ. (United States)
Shalini Jain, The Univ. of Texas M.D. Anderson Cancer Ctr. (United States)
Xiao Wang, The Univ. of Texas M.D. Anderson Cancer Ctr. (United States)
Dihua Yu, The Univ. of Texas M.D. Anderson Cancer Ctr. (United States)
Rebecca R. Richards-Kortum, Rice Univ. (United States)
Tomasz S. Tkaczyk, Rice Univ. (United States)

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