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Journal of Biomedical Optics

In situ histology of mice skin through transfer learning of tissue energy interaction in optical coherence tomography
Author(s): Debdoot Sheet; Amrita Chaudhary; Sri Phani Krishna Karri; Debnath Das; Amin Katouzian; Provas Banerjee; Nassir Navab; Jyotirmoy Chatterjee; Ajoy K. Ray
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Paper Abstract

Tissue characterization method in optical coherence tomography (OCT) for in situ histology of soft tissues is presented and demonstrated for mice skin. OCT allows direct noninvasive visualization of subsurface anatomy. It is currently used for in situ investigation of lesions in skin, vessels, retinal layers, oral, and bronchial cavitities. Although OCT images present high resolution information about tissue morphology, reporting requires a reader experienced in interpretation of the images, viz., identification of anatomical layers and structures constituting the organ based on OCT speckle appearance. Our approach characterizes tissues through transfer learning of tissue energy interaction statistical physics models of ballistic and near-ballistic photons. The clinical information yield with our approach is comparable to conventional invasive histology. On cross evaluation with a mice model experiment, the epidermis, papillary dermis, dermis, and adipose tissue constituting the mice skin are identified with an accuracy of 99%, 95%, 99%, and 98%, respectively. This high accuracy of characterizing heterogeneous tissues using OCT justifies the ability of our computational approach to perform in situ histology and can be extended to regular clinical practice for diagnosis of vascular, retinal, or oral pathologies.

Paper Details

Date Published: 25 September 2013
PDF: 4 pages
J. Biomed. Opt. 18(9) 090503 doi: 10.1117/1.JBO.18.9.090503
Published in: Journal of Biomedical Optics Volume 18, Issue 9
Show Author Affiliations
Debdoot Sheet, Indian Institute of Technology Kharagpur (India)
Amrita Chaudhary, Indian Institute of Technology Kharagpur (India)
Sri Phani Krishna Karri, Indian Institute of Technology Kharagpur (India)
Debnath Das, Indian Institute of Technology Kharagpur (India)
Amin Katouzian, Technische Univ. München (Germany)
Provas Banerjee, Banerjee's Biomedical Research Foundation (India)
Nassir Navab, Technische Univ. München (Germany)
Jyotirmoy Chatterjee, Indian Institute of Technology Kharagpur (India)
Ajoy K. Ray, Indian Institute of Technology Kharagpur (India)

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