Share Email Print
cover

Journal of Biomedical Optics • Open Access

Laser speckle contrast imaging of blood flow in rat retinas using an endoscope
Author(s): Adrien Ponticorvo; Damon Cardenas; Andrew K. Dunn; Daniel Ts'o; Timothy Q. Duong

Paper Abstract

Laser speckle contrast imaging (LSCI) offers a cost-effective means to image blood flow in vivo. However, it is not commonly used to image rodent retinas because of the challenges associated with imaging through the curved cornea and delivering light through the highly scattering lens. A solution to overcome these problems by using LSCI in conjunction with an endoscope to obtain high spatiotemporal blood flow images is described. Its utility is demonstrated by imaging blood flow changes in rat retinas using hyperoxic, hypercapnic, and visual (flicker) stimulations. Hypercapnia increases blood flow, hyperoxia decreases blood flow, and visual stimulation increases blood flow in the retina relative to basal conditions. The time-to-peak of the LSCI response to visual stimulation is also measured. This approach may prove useful to investigate dysregulation in blood flow-evoked responses in retinal diseases and to evaluate treatment strategies in rodents.

Paper Details

Date Published: 24 September 2013
PDF: 4 pages
J. Biomed. Opt. 18(9) 090501 doi: 10.1117/1.JBO.18.9.090501
Published in: Journal of Biomedical Optics Volume 18, Issue 9
Show Author Affiliations
Adrien Ponticorvo, The Univ. of Texas Health Science Ctr. at San Antonio (United States)
Damon Cardenas, The Univ. of Texas Health Science Ctr. at San Antonio (United States)
Andrew K. Dunn, The Univ. of Texas at Austin (United States)
Daniel Ts'o, SUNY Upstate Medical Univ. (United States)
Timothy Q. Duong, The Univ. of Texas Health Science Ctr. at San Antonio (United States)


© SPIE. Terms of Use
Back to Top