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Journal of Biomedical Optics

Native fluorescence spectroscopy reveals spectral differences among prostate cancer cell lines with different risk levels
Author(s): Yang Pu; Jianpeng Xue; Wubao Wang; Baogang Xu; Yueqing Gu; Rui Tang; Ellen Ackerstaff; Jason A. Koutcher; Samuel Achilefu; Robert R. Alfano
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Paper Abstract

The spectral changes of native fluorophores among normal fibroblasts and cancer cell lines of different metastatic ability are investigated by fluorescence spectroscopy. The normal (fibroblast), moderately metastatic (DU-145), and advanced metastatic (PC-3) cell lines were each selectively excited at 300 nm, and their fluorescence emission spectra are analyzed using principal component analysis to explore the differences of the relative contents of tryptophan and reduced nicotinamide adenine dinucleotide in these cell lines. The results show that the tryptophan emission featured predominantly in the fluorescence spectra of the advanced metastatic cancer cells in comparison with the moderately metastatic cancer and normal cells.

Paper Details

Date Published: 2 August 2013
PDF: 6 pages
J. Biomed. Opt. 18(8) 087002 doi: 10.1117/1.JBO.18.8.087002
Published in: Journal of Biomedical Optics Volume 18, Issue 8
Show Author Affiliations
Yang Pu, The City College of New York (United States)
Jianpeng Xue, China Pharmaceutical Univ. (China)
Wubao Wang, The City College of New York (United States)
Baogang Xu, Washington Univ. in St. Louis (United States)
Yueqing Gu, China Pharmaceutical Univ. (China)
Rui Tang, Washington Univ. in St. Louis (United States)
Ellen Ackerstaff, Memorial Sloan-Kettering Cancer Ctr. (United States)
Jason A. Koutcher, Memorial Sloan-Kettering Cancer Ctr. (United States)
Samuel Achilefu, Washington Univ. School of Medicine in St. Louis (United States)
Robert R. Alfano, The City College of New York (United States)

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