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Journal of Biomedical Optics

Mitigating fluorescence spectral overlap in wide-field endoscopic imaging
Author(s): Chenying Yang; Vivian W. Hou; Leonard Y. Nelson; Eric J. Seibel
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Paper Abstract

The number of molecular species suitable for multispectral fluorescence imaging is limited due to the overlap of the emission spectra of indicator fluorophores, e.g., dyes and nanoparticles. To remove fluorophore emission cross-talk in wide-field multispectral fluorescence molecular imaging, we evaluate three different solutions: (1) image stitching, (2) concurrent imaging with cross-talk ratio subtraction algorithm, and (3) frame-sequential imaging. A phantom with fluorophore emission cross-talk is fabricated, and a 1.2-mm ultrathin scanning fiber endoscope (SFE) is used to test and compare these approaches. Results show that fluorophore emission cross-talk could be successfully avoided or significantly reduced. Near term, the concurrent imaging method of wide-field multispectral fluorescence SFE is viable for early stage cancer detection and localization in vivo. Furthermore, a means to enhance exogenous fluorescence target-to-background ratio by the reduction of tissue autofluorescence background is demonstrated.

Paper Details

Date Published: 21 August 2013
PDF: 14 pages
J. Biomed. Opt. 18(8) 086012 doi: 10.1117/1.JBO.18.8.086012
Published in: Journal of Biomedical Optics Volume 18, Issue 8
Show Author Affiliations
Chenying Yang, Univ. of Washington (United States)
Vivian W. Hou, Univ. of Washington (United States)
Leonard Y. Nelson, Univ. of Washington (United States)
Eric J. Seibel, Univ. of Washington (United States)


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