Share Email Print

Journal of Biomedical Optics • Open Access

Blind unmixing of spectrally resolved lifetime images
Author(s): Farzad Fereidouni; Gerhard A. Blab; Hans C. Gerritsen

Paper Abstract

A method, is presented for blind unmixing spectrally resolved fluorescence lifetime images. The method is based on the combined analysis of spectral and lifetime phasors and allows unmixing of up to three components without any prior knowledge. Fractional intensities, spectra and decay curves of the individual components can be extracted with this new technique. The reliability and sensitivity are investigated and the possibility of extending the method to unmix more components is discussed. The method is evaluated on mixtures of fluorescent dyes and labeled cells.

Paper Details

Date Published: 12 August 2013
PDF: 13 pages
J. Biomed. Opt. 18(8) 086006 doi: 10.1117/1.JBO.18.8.086006
Published in: Journal of Biomedical Optics Volume 18, Issue 8
Show Author Affiliations
Farzad Fereidouni, Utrecht Univ. (Netherlands)
Gerhard A. Blab, Utrecht Univ. (Netherlands)
Hans C. Gerritsen, Utrecht Univ. (Netherlands)

© SPIE. Terms of Use
Back to Top