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Journal of Biomedical Optics

Optimized goniometer for determination of the scattering phase function of suspended particles: simulations and measurements
Author(s): Florian Foschum; Alwin Kienle
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Paper Abstract

We present simulations and measurements with an optimized goniometer for determination of the scattering phase function of suspended particles. We applied the Monte Carlo method, using a radially layered cylindrical geometry and mismatched boundary conditions, in order to investigate the influence of reflections caused by the interfaces of the glass cuvette and the scatterer concentration on the accurate determination of the scattering phase function. Based on these simulations we built an apparatus which allows direct measurement of the phase function from ϑ=7  deg to ϑ=172  deg without any need for correction algorithms. Goniometric measurements on polystyrene and SiO2 spheres proved this concept. Using the validated goniometer, we measured the phase function of yeast cells, demonstrating the improvement of the new system compared to standard goniometers. Furthermore, the scattering phase function of different fat emulsions, like Intralipid, was determined precisely.

Paper Details

Date Published: 23 August 2013
PDF: 7 pages
J. Biomed. Opt. 18(8) 085002 doi: 10.1117/1.JBO.18.8.085002
Published in: Journal of Biomedical Optics Volume 18, Issue 8
Show Author Affiliations
Florian Foschum, Univ. Ulm (Germany)
Alwin Kienle, Univ. Ulm (Germany)

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