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Journal of Biomedical Optics

In vivo photoacoustic lifetime imaging of tumor hypoxia in small animals
Author(s): Qi Shao; Ekaterina Morgounova; Chunlan Jiang; Jeung-Hwan Choi; John C. Bischof; Shai Ashkenazi
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Paper Abstract

Tumor hypoxia is an important factor in assessment of both cancer progression and cancer treatment efficacy. This has driven a substantial effort toward development of imaging modalities that can directly measure oxygen distribution and therefore hypoxia in tissue. Although several approaches to measure hypoxia exist, direct measurement of tissue oxygen through an imaging approach is still an unmet need. To address this, we present a new approach based on in vivo application of photoacoustic lifetime imaging (PALI) to map the distribution of oxygen partial pressure (pO2 ) in tissue. This method utilizes methylene blue, a dye widely used in clinical applications, as an oxygen-sensitive imaging agent. PALI measurement of oxygen relies upon pO2 -dependent excitation lifetime of the dye. A multimodal imaging system was designed and built to achieve ultrasound (US), photoacoustic, and PALI imaging within the same system. Nude mice bearing LNCaP xenograft hindlimb tumors were used as the target tissue. Hypoxic regions were identified within the tumor in a combined US/PALI image. Finally, the statistical distributions of pO2 in tumor, normal, and control tissues were compared with measurements by a needle-mounted oxygen probe. A statistically significant drop in mean pO2 was consistently detected by both methods in tumors.

Paper Details

Date Published: 22 July 2013
PDF: 8 pages
J. Biomed. Opt. 18(7) 076019 doi: 10.1117/1.JBO.18.7.076019
Published in: Journal of Biomedical Optics Volume 18, Issue 7
Show Author Affiliations
Qi Shao, Univ. of Minnesota (United States)
Ekaterina Morgounova, Univ. of Minnesota (United States)
Chunlan Jiang
Jeung-Hwan Choi
John C. Bischof, Univ. of Minnesota, Twin Cities (United States)
Shai Ashkenazi, Univ. of Minnesota (United States)

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