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Journal of Biomedical Optics • Open Access

Rapid diagnosis of liver fibrosis using multimodal multiphoton nonlinear optical microspectroscopy imaging
Author(s): Jang Hyuk Lee; Jong Chul Kim; Giyoong Tae; Myoung-kyu Oh; Do-Kyeong Ko

Paper Abstract

A multimodal multiphoton nonlinear optical (NLO) microspectroscopy imaging system was developed using a femtosecond laser and a photonic crystal fiber. Coherent anti-Stokes Raman scattering (CARS) microspectroscopy was combined with two-photon excitation fluorescence and second-harmonic generation microscopy in one platform and the system was applied to diagnose liver fibrosis. Normal and liver fibrosis tissues were clearly distinguished with the great difference from CARS spectra as well as multimodal multiphoton NLO images. We expect the system to be a rapid diagnosis tool for liver fibrosis at tissue level with label-free imaging of significant biochemical components.

Paper Details

Date Published: 9 July 2013
PDF: 7 pages
J. Biomed. Opt. 18(7) 076009 doi: 10.1117/1.JBO.18.7.076009
Published in: Journal of Biomedical Optics Volume 18, Issue 7
Show Author Affiliations
Jang Hyuk Lee, Gwangju Institute of Science and Technology (Korea, Republic of)
Jong Chul Kim, Gwangju Institute of Science and Technology (Korea, Republic of)
Giyoong Tae, Gwangju Institute of Science and Technology (Korea, Republic of)
Myoung-kyu Oh, Gwangju Institute of Science and Technology (Korea, Republic of)
Do-Kyeong Ko, Gwangju Institute of Science and Technology (Korea, Republic of)


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