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Journal of Biomedical Optics

High-throughput fabrication of gray-level biomicrostructures via temporal focusing excitation and laser pulse control
Author(s): Yi-Cheng Li; Li-Chung Cheng; Chia-Yuan Chang; Chun-Yu Lin; Nan-Shan Chang; Paul J. Campagnola; Chen-Yuan Dong; Shean-Jen Chen
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Paper Abstract

A developed temporal focusing-based multiphoton excitation system with additional patterned excitation and local laser control can provide high-throughput fabrication of three-dimensional gray-level biomicrostructures via two-photon cross-linking with rose bengal (RB) as the photoactivator. Multiple bovine serum albumin (BSA) structures of different concentrations were simultaneously achieved by selecting different pulse numbers in the designated regions with an appropriate femtosecond laser power within a few seconds. Intensity of the RB two-photon excited fluorescence (TPEF) is correlated to the concentration of the fabricated cross-linked BSA microstructure. Hence, the fabricated BSA microstructure can be monitored online by utilizing the RB TPEF as contrast agent. It suggests that this approach with its unique capability of high-speed, gray-level, and online-inspection fabrication meets the requirements of the biomedical researches involved in extracellular matrix.

Paper Details

Date Published: 25 July 2013
PDF: 6 pages
J. Biomed. Opt. 18(7) 075004 doi: 10.1117/1.JBO.18.7.075004
Published in: Journal of Biomedical Optics Volume 18, Issue 7
Show Author Affiliations
Yi-Cheng Li, National Cheng Kung Univ. (Taiwan)
Li-Chung Cheng, National Cheng Kung Univ. (Taiwan)
Chia-Yuan Chang, National Cheng Kung Univ. (Taiwan)
Chun-Yu Lin, National Cheng Kung Univ. (Taiwan)
Nan-Shan Chang, National Cheng Kung Univ. (Taiwan)
Paul J. Campagnola, Univ. of Wisconsin-Madison (United States)
Chen-Yuan Dong, National Taiwan Univ. (Taiwan)
Shean-Jen Chen, National Cheng Kung Univ. (Taiwan)

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