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Journal of Biomedical Optics

Relationship between the chemical and morphological characteristics of human dentin after Er:YAG laser irradiation
Author(s): Luis Eduardo Silva Soares; Ovidio Cesar Lavesa Martin; Lilian T. Moriyama; Cristina Kurachi; Airton A. Martin
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Paper Abstract

The effects of laser etching on dentin are studied by microenergy-dispersive x-ray fluorescence spectrometry (μ-EDXRF) and scanning electron microscopy (SEM) to establish the correlation of data obtained. Fifteen human third molars are prepared, baseline μ-EDXRF mappings are performed, and ten specimens are selected. Each specimen received four treatments: acid etching (control-CG) or erbium:yttrium–aluminum–garnet (Er:YAG) laser irradiation (I—100 mJ, II—160 mJ, and III—220 mJ), and maps are done again. The Ca and P content are significantly reduced after acid etching (p<0.0001 ) and increased after laser irradiation with 220 mJ (Ca: p<0.0153 and P: p=0.0005 ). The Ca/P ratio increased and decreased after CG (p=0.0052 ) and GI (p=0.0003 ) treatments, respectively. CG treatment resulted in lower inorganic content (GI: p<0.05 , GII: p<0.01 , and GIII: p<;0.01 ) and higher Ca/P ratios than laser etching (GI: p<;0.001 , GII: p<0.01 , and GIII: p<0.01 ). The SEM photomicrographies revealed open (CG) and partially open dentin tubules (GI, GII, and GIII). μ-EDXRF mappings illustrated that acid etching created homogeneous distribution of inorganic content over dentin. Er:YAG laser etching (220 mJ) produced irregular elemental distribution and changed the stoichiometric proportions of hydroxyapatite, as showed by an increase of mineral content. Decreases and increases of mineral content in the μ-EDXRF images are correlated to holes and mounds, respectively, as found in SEM images.

Paper Details

Date Published: 24 June 2013
PDF: 9 pages
J. Biomed. Opt. 18(6) 068001 doi: 10.1117/1.JBO.18.6.068001
Published in: Journal of Biomedical Optics Volume 18, Issue 6
Show Author Affiliations
Luis Eduardo Silva Soares, Univ. do Vale do Paraíba (Brazil)
Ovidio Cesar Lavesa Martin, Univ. do Vale do Paraíba (Brazil)
Lilian T. Moriyama, Univ. de São Paulo (Brazil)
Cristina Kurachi, Univ. de São Paulo (Brazil)
Airton A. Martin, Univ. do Vale do Paraíba (Brazil)

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