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Journal of Biomedical Optics • Open Access

Near-infrared-excited confocal Raman spectroscopy advances in vivo diagnosis of cervical precancer
Author(s): Shiyamala Duraipandian; Wei Zheng; Joseph Ng; Jeffrey J. Low; Arunachalam Ilancheran; Zhiwei Huang

Paper Abstract

Raman spectroscopy is a unique optical technique that can probe the changes of vibrational modes of biomolecules associated with tissue premalignant transformation. This study evaluates the clinical utility of confocal Raman spectroscopy over near-infrared (NIR) autofluorescence (AF) spectroscopy and composite NIR AF/Raman spectroscopy for improving early diagnosis of cervical precancer in vivo at colposcopy. A rapid NIR Raman system coupled with a ball-lens fiber-optic confocal Raman probe was utilized for in vivo NIR AF/Raman spectral measurements of the cervix. A total of 1240 in vivo Raman spectra [normal (n=993 ), dysplasia (n=247 )] were acquired from 84 cervical patients. Principal components analysis (PCA) and linear discriminant analysis (LDA) together with a leave-one-patient-out, cross-validation method were used to extract the diagnostic information associated with distinctive spectroscopic modalities. The diagnostic ability of confocal Raman spectroscopy was evaluated using the PCA-LDA model developed from the significant principal components (PCs) [i.e., PC4, 0.0023%; PC5, 0.00095%; PC8, 0.00022%, (p<0.05 )], representing the primary tissue Raman features (e.g., 854, 937, 1095, 1253, 1311, 1445, and 1654  cm −1 ). Confocal Raman spectroscopy coupled with PCA-LDA modeling yielded the diagnostic accuracy of 84.1% (a sensitivity of 81.0% and a specificity of 87.1%) for in vivo discrimination of dysplastic cervix. The receiver operating characteristic curves further confirmed that the best classification was achieved using confocal Raman spectroscopy compared to the composite NIR AF/Raman spectroscopy or NIR AF spectroscopy alone. This study illustrates that confocal Raman spectroscopy has great potential to improve early diagnosis of cervical precancer in vivo during clinical colposcopy.

Paper Details

Date Published: 24 June 2013
PDF: 8 pages
J. Biomed. Opt. 18(6) 067007 doi: 10.1117/1.JBO.18.6.067007
Published in: Journal of Biomedical Optics Volume 18, Issue 6
Show Author Affiliations
Shiyamala Duraipandian, National Univ. of Singapore (Singapore)
Wei Zheng, National Univ. of Singapore (Singapore)
Joseph Ng, National Univ. Hospital (Singapore)
Jeffrey J. Low, National Univ. Hospital (Singapore)
Arunachalam Ilancheran, National Univ. Hospital (Singapore)
Zhiwei Huang, National Univ. of Singapore (Singapore)


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