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Journal of Biomedical Optics • Open Access

Video-rate imaging of microcirculation with single-exposure oblique back-illumination microscopy

Paper Abstract

Oblique back-illumination microscopy (OBM) is a new technique for simultaneous, independent measurements of phase gradients and absorption in thick scattering tissues based on widefield imaging. To date, OBM has been used with sequential camera exposures, which reduces temporal resolution, and can produce motion artifacts in dynamic samples. Here, a variation of OBM that allows single-exposure operation with wavelength multiplexing and image splitting with a Wollaston prism is introduced. Asymmetric anamorphic distortion induced by the prism is characterized and corrected in real time using a graphics-processing unit. To demonstrate the capacity of single-exposure OBM to perform artifact-free imaging of blood flow, video-rate movies of microcirculation in ovo in the chorioallantoic membrane of the developing chick are presented. Imaging is performed with a high-resolution rigid Hopkins lens suitable for endoscopy.

Paper Details

Date Published: 3 June 2013
PDF: 9 pages
J. Biomed. Opt. 18(6) 066007 doi: 10.1117/1.JBO.18.6.066007
Published in: Journal of Biomedical Optics Volume 18, Issue 6
Show Author Affiliations
Tim N. Ford, Boston Univ. (United States)
Jerome Mertz, Boston Univ. (United States)

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