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Journal of Biomedical Optics • Open Access

Optimizing the performance of dual-axis confocal microscopes via Monte-Carlo scattering simulations and diffraction theory
Author(s): Ye Chen; Jonathan T. Liu

Paper Abstract

Dual-axis confocal (DAC) microscopy has been found to exhibit superior rejection of out-of-focus and multiply scattered background light compared to conventional single-axis confocal microscopy. DAC microscopes rely on the use of separated illumination and collection beam paths that focus and intersect at a single focal volume (voxel) within tissue. While it is generally recognized that the resolution and contrast of a DAC microscope depends on both the crossing angle of the DAC beams,, and the focusing numerical aperture of the individual beams, α , a detailed study to investigate these dependencies has not been performed. Contrast and resolution are considered as two main criteria to assess the performance of a point-scanned DAC microscope (DAC-PS) and a line-scanned DAC microscope (DAC-LS) as a function of θ and α . The contrast and resolution of these designs are evaluated by Monte-Carlo scattering simulations and diffraction theory calculations, respectively. These results can be used for guiding the optimal designs of DAC-PS and DAC-LS microscopes.

Paper Details

Date Published: 3 June 2013
PDF: 5 pages
J. Biomed. Opt. 18(6) 066006 doi: 10.1117/1.JBO.18.6.066006
Published in: Journal of Biomedical Optics Volume 18, Issue 6
Show Author Affiliations
Ye Chen, Stony Brook Univ. (United States)
Jonathan T. Liu, Stony Brook Univ. (United States)

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