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Journal of Biomedical Optics • Open Access

Dual/differential coherent anti-Stokes Raman scattering module for multiphoton microscopes with a femtosecond Ti:sapphire oscillator
Author(s): Bei Li; Paola Borri; Wolfgang Langbein

Paper Abstract

In the last decade, coherent anti-Stokes Raman scattering (CARS) microscopy has emerged as a powerful multiphoton imaging technique offering label-free chemical sensitivity and high three-dimensional resolution. However, its widespread application in the life sciences has been hampered by the use of costly pulsed lasers, the existence of a nonresonant background requiring involved technical solutions for its efficient suppression, and the limited acquisition speed of multiplex techniques addressing several vibrational resonances, if improved chemical specificity is needed. We have recently reported a differential CARS technique (D-CARS), which simultaneously measures two vibrational frequencies, enhancing the chemical selectivity and sensitivity without introducing costly hardware, while maintaining fast acquisition. In this study, we demonstrate a compact, fully automated, cost-effective module, which integrates on hardware and software level with a commercial multiphoton microscope based on a single 100 fs Ti:Sapphire oscillator and enables D-CARS microscopy in a user-friendly format for applications in the life sciences.

Paper Details

Date Published: 3 June 2013
PDF: 6 pages
J. Biomed. Opt. 18(6) 066004 doi: 10.1117/1.JBO.18.6.066004
Published in: Journal of Biomedical Optics Volume 18, Issue 6
Show Author Affiliations
Bei Li, Cardiff Univ. (United Kingdom)
Paola Borri, Cardiff Univ. (United Kingdom)
Wolfgang Langbein, Cardiff Univ. (United Kingdom)

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