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Journal of Biomedical Optics • Open Access

In vivo visualization of dermal collagen fiber in skin burn by collagen-sensitive second-harmonic-generation microscopy
Author(s): Ryosuke Tanaka; Shu-ichiro Fukushima; Kunihiko Sasaki; Yuji Tanaka; Hiroyuki Murota; Kenji Matsumoto; Tsutomu Araki; Takeshi Yasui

Paper Abstract

Optical assessment of skin burns is possible with second-harmonic-generation (SHG) microscopy due to its high sensitivity to thermal denaturation of collagen molecules. In contrast to previous studies that were performed using excised tissue specimens ex vivo, in vivo observation of dermal collagen fibers in living rat burn models with SHG microscopy is demonstrated. Changes in signal vanishing patterns in the SHG images are confirmed to be dependent on the burn degree. Comparison of the SHG images with Masson’s trichrome-stained images indicated that the observed patterns were caused by the coexistence of molten and fibrous structures of dermal collagen fibers. Furthermore, a quantitative parameter for burn assessment based on the depth profile of the mean SHG intensity across the entire SHG image is proposed. These results and discussions imply a potential of SHG microscopy as a minimally invasive, highly quantitative tool for skin burn assessment.

Paper Details

Date Published: 12 April 2013
PDF: 9 pages
J. Biomed. Opt. 18(6) 061231 doi: 10.1117/1.JBO.18.6.061231
Published in: Journal of Biomedical Optics Volume 18, Issue 6
Show Author Affiliations
Ryosuke Tanaka, Osaka Univ. (Japan)
Shu-ichiro Fukushima, Osaka Univ. (Japan)
Kunihiko Sasaki, Osaka Univ. (Japan)
Yuji Tanaka, Osaka Univ. (Japan)
Hiroyuki Murota, Osaka Univ. Graduate School of Medicine, FOM (Japan)
Kenji Matsumoto, Osaka Univ. (Japan)
Tsutomu Araki, Osaka Univ. (Japan)
Takeshi Yasui, Osaka Univ. (Japan)

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