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Journal of Biomedical Optics • Open Access

Confocal microscopy with strip mosaicing for rapid imaging over large areas of excised tissue
Author(s): Sanjeewa Abeytunge; Yongbiao Li; Bjorg A. Larson; Gary Peterson; Emily Seltzer; Ricardo Toledo-Crow; Milind Rajadhyaksha

Paper Abstract

Confocal mosaicing microscopy is a developing technology platform for imaging tumor margins directly in freshly excised tissue, without the processing required for conventional pathology. Previously, mosaicing on 12-×-12  mm 2 of excised skin tissue from Mohs surgery and detection of basal cell carcinoma margins was demonstrated in 9 min. Last year, we reported the feasibility of a faster approach called “strip mosaicing,” which was demonstrated on a 10-×-10  mm 2 of tissue in 3 min. Here we describe further advances in instrumentation, software, and speed. A mechanism was also developed to flatten tissue in order to enable consistent and repeatable acquisition of images over large areas. We demonstrate mosaicing on 10-×-10  mm 2 of skin tissue with 1-μm lateral resolution in 90 s. A 2.5-×-3.5  cm 2 piece of breast tissue was scanned with 0.8-μm lateral resolution in 13 min. Rapid mosaicing of confocal images on large areas of fresh tissue potentially offers a means to perform pathology at the bedside. Imaging of tumor margins with strip mosaicing confocal microscopy may serve as an adjunct to conventional (frozen or fixed) pathology for guiding surgery.

Paper Details

Date Published: 6 February 2013
PDF: 14 pages
J. Biomed. Opt. 18(6) 061227 doi: 10.1117/1.JBO.18.6.061227
Published in: Journal of Biomedical Optics Volume 18, Issue 6
Show Author Affiliations
Sanjeewa Abeytunge, Memorial Sloan-Kettering Cancer Ctr. (United States)
Yongbiao Li, Memorial Sloan-Kettering Cancer Ctr. (United States)
Bjorg A. Larson, Memorial Sloan-Kettering Cancer Ctr. (United States)
Gary Peterson, Memorial Sloan-Kettering Cancer Ctr. (United States)
Emily Seltzer, Livingston High School (United States)
Ricardo Toledo-Crow, Memorial Sloan-Kettering Cancer Ctr. (United States)
Milind Rajadhyaksha, Memorial Sloan-Kettering Cancer Ctr. (United States)


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