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Journal of Biomedical Optics

Stepwise multiphoton activation fluorescence reveals a new method of melanin detection
Author(s): Zhenhua Lai; Josef Kerimo; Yair J. Mega; Charles A. DiMarzio
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Paper Abstract

The stepwise multiphoton activated fluorescence (SMPAF) of melanin, activated by a continuous-wave mode near infrared (NIR) laser, reveals a broad spectrum extending from the visible spectra to the NIR and has potential application for a low-cost, reliable method of detecting melanin. SMPAF images of melanin in mouse hair and skin are compared with conventional multiphoton fluorescence microscopy and confocal reflectance microscopy (CRM). By combining CRM with SMPAF, we can locate melanin reliably. However, we have the added benefit of eliminating background interference from other components inside mouse hair and skin. The melanin SMPAF signal from the mouse hair is a mixture of a two-photon process and a third-order process. The melanin SMPAF emission spectrum is activated by a 1505.9-nm laser light, and the resulting spectrum has a peak at 960 nm. The discovery of the emission peak may lead to a more energy-efficient method of background-free melanin detection with less photo-bleaching.

Paper Details

Date Published: 7 January 2013
PDF: 8 pages
J. Biomed. Opt. 18(6) 061225 doi: 10.1117/1.JBO.18.6.061225
Published in: Journal of Biomedical Optics Volume 18, Issue 6
Show Author Affiliations
Zhenhua Lai, Northeastern Univ. (United States)
Josef Kerimo, Northeastern Univ. (United States)
Yair J. Mega, Northeastern Univ. (United States)
Charles A. DiMarzio, Northeastern Univ. (United States)

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