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Journal of Biomedical Optics

Setup for investigating gold nanoparticle penetration through reconstructed skin and comparison to published human skin data
Author(s): Hagar I. Labouta; Sibylle Thude; Marc Schneider
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Paper Abstract

Owing to the limited source of human skin (HS) and the ethical restrictions of using animals in experiments, in vitro skin equivalents are a possible alternative for conducting particle penetration experiments. The conditions for conducting penetration experiments with model particles, 15-nm gold nanoparticles (AuNP), through nonsealed skin equivalents are described for the first time. These conditions include experimental setup, sterility conditions, effective applied dose determination, skin sectioning, and skin integrity check. Penetration at different exposure times (two and 24 h) and after tissue fixation (fixed versus unfixed skin) are examined to establish a benchmark in comparison to HS in an attempt to get similar results to HS experiments presented earlier. Multiphoton microscopy is used to detect gold luminescence in skin sections. λex = 800 nm is used for excitation of AuNP and skin samples, allowing us to determine a relative index for particle penetration. Despite the observed overpredictability of penetration into skin equivalents, they could serve as a first fast screen for testing the behavior of nanoparticles and extrapolate their penetration behavior into HS. Further investigations are required to test a wide range of particles of different physicochemical properties to validate the skin equivalent-human skin particle penetration relationship.

Paper Details

Date Published: 30 November 2012
PDF: 8 pages
J. Biomed. Opt. 18(6) 061218 doi: 10.1117/1.JBO.18.6.061218
Published in: Journal of Biomedical Optics Volume 18, Issue 6
Show Author Affiliations
Hagar I. Labouta, Alexandria Univ. (Egypt)
Sibylle Thude, Fraunhofer-Institut für Grenzflächen- und Bioverfahrenstechnik (Germany)
Marc Schneider, Univ. des Saarlandes (Germany)


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