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Journal of Biomedical Optics

Background free imaging of upconversion nanoparticle distribution in human skin
Author(s): Zhen Song; Yuri G. Anissimov; Jiangbo Zhao; Andrei V. Nechaev; Annemarie Nadort; Dayong Jin; Tarl W. Prow; Michael S. Roberts; Andrei V. Zvyagin
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Paper Abstract

Widespread applications of nanotechnology materials have raised safety concerns due to their possible penetration through skin and concomitant uptake in the organism. This calls for systematic study of nanoparticle transport kinetics in skin, where high-resolution optical imaging approaches are often preferred. We report on application of emerging luminescence nanomaterial, called upconversion nanoparticles (UCNPs), to optical imaging in skin that results in complete suppression of background due to the excitation light back-scattering and biological tissue autofluorescence. Freshly excised intact and microneedle-treated human skin samples were topically coated with oil formulation of UCNPs and optically imaged. In the first case, 8- and 32-nm UCNPs stayed at the topmost layer of the intact skin, stratum corneum. In the second case, 8-nm nanoparticles were found localized at indentations made by the microneedle spreading in dermis very slowly (estimated diffusion coefficient, Dnp=3-7×10−12  cm2·s−1). The maximum possible UCNP-imaging contrast was attained by suppressing the background level to that of the electronic noise, which was estimated to be superior in comparison with the existing optical labels.

Paper Details

Date Published: 27 November 2012
PDF: 10 pages
J. Biomed. Opt. 18(6) 061215 doi: 10.1117/1.JBO.18.6.061215
Published in: Journal of Biomedical Optics Volume 18, Issue 6
Show Author Affiliations
Zhen Song, Macquarie Univ. (Australia)
Yuri G. Anissimov, Griffith Univ. (Australia)
Jiangbo Zhao, Macquarie Univ. (Australia)
Andrei V. Nechaev, Moscow Lomonosov State Academy of Fine Chemical Technology (Russian Federation)
Annemarie Nadort, Academisch Medisch Ctr. (Netherlands)
Dayong Jin, Macquarie Univ. (Australia)
Tarl W. Prow, The Univ. of Queensland School of Medicine (Australia)
Michael S. Roberts, The Univ. of Queensland School of Medicine (Australia)
Andrei V. Zvyagin, Macquarie Univ. (Australia)

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