Share Email Print

Journal of Biomedical Optics

Switchable skin window induced by optical clearing method for dermal blood flow imaging
Author(s): Jing Wang; Rui Shi; Dan Zhu
Format Member Price Non-Member Price
PDF $20.00 $25.00
cover GOOD NEWS! Your organization subscribes to the SPIE Digital Library. You may be able to download this paper for free. Check Access

Paper Abstract

Optical imaging techniques have shown tremendous potential for assessing cutaneous microcirculation, but the imaging depth and contrast is limited by the strong scattering of skin. Current skin windows have to be fulfilled by surgical operation and suffer from some side effects. In this study, a switchable skin window was developed by topical application of an optical clearing agent (OCA) and saline on rat skin in vivo. The validity of the skin window was evaluated by the laser speckle contrast imaging technique, and the safety of OCA to the body was tested through histologic examinations. The results indicated that administration of OCA or saline on rat skin in vivo can open or close the window of skin repeatedly for three days. With the repair effect of hyaluronic acid and Vaseline, it is able to repeatedly visualize the dermal blood vessels and flow distribution. Long-term observation shows that there is no abnormal reflection in micro-structure, body weight, organ coefficients, histopathologic lesions, or toxic reactions compared with a control group. This switchable window will provide an effective tool not only for cutaneous microcirculation with laser speckle contrast imaging, but also for diagnosis and treatment of peripheral vascular diseases, including tumor research with various optical imaging techniques.

Paper Details

Date Published: 15 November 2012
PDF: 6 pages
J. Biomed. Opt. 18(6) 061209 doi: 10.1117/1.JBO.18.6.061209
Published in: Journal of Biomedical Optics Volume 18, Issue 6
Show Author Affiliations
Jing Wang, Britton Chance Ctr. for Biomedical Photonics (China)
Rui Shi, Britton Chance Ctr. for Biomedical Photonics (China)
Dan Zhu, Britton Chance Ctr. for Biomedical Photonics (China)

© SPIE. Terms of Use
Back to Top