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Journal of Biomedical Optics

Vibrational spectroscopy and microscopic imaging: novel approaches for comparing barrier physical properties in native and human skin equivalents
Author(s): Guo Yu; Guojin Zhang; Carol R. Flach; Richard Mendelsohn
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Paper Abstract

Vibrational spectroscopy and imaging have been used to compare barrier properties in human skin, porcine skin, and two human skin equivalents, Epiderm 200X with an enhanced barrier and Epiderm 200 with a normal barrier. Three structural characterizations were performed. First, chain packing and conformational order were compared in isolated human stratum corneum (SC), isolated porcine SC, and in the Epiderm 200X surface layers. The infrared (IR) spectrum of isolated human SC revealed a large proportion of orthorhombically packed lipid chains at physiological temperatures along with a thermotropic phase transition to a state with hexagonally packed chains. In contrast, the lipid phase at physiological temperatures in both porcine SC and in Epiderm 200X, although dominated by conformationally ordered chains, lacked significant levels of orthorhombic subcell packing. Second, confocal Raman imaging of cholesterol bands showed extensive formation of cholesterol-enriched pockets within the human skin equivalents (HSEs). Finally, IR imaging tracked lipid barrier dimensions as well as the spatial disposition of ordered lipids in human SC and Epiderm 200X. These approaches provide a useful set of experiments for exploring structural differences between excised human skin and HSEs, which in turn may provide a rationale for the functional differences observed among these preparations.

Paper Details

Date Published: 19 November 2012
PDF: 11 pages
J. Biomed. Opt. 18(6) 061207 doi: 10.1117/1.JBO.18.6.061207
Published in: Journal of Biomedical Optics Volume 18, Issue 6
Show Author Affiliations
Guo Yu, Rutgers, The State Univ. of New Jersey (United States)
Guojin Zhang, International Specialty Products, Inc. (United States)
Carol R. Flach, Rutgers, The State Univ. of New Jersey (United States)
Richard Mendelsohn, Rutgers, The State Univ. of New Jersey (United States)


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