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Journal of Biomedical Optics

Combined spectrophotometry and tensile measurements of human connective tissues: potentials and limitations
Author(s): Markus Ernstberger; Freddy Sichting; Tobias Baselt; Peter Hartmann; Gabriela Aust; Niels Hammer
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Paper Abstract

Strain-dependent transmission data of nine iliotibial tract specimens are determined using a custom-built optical setup with a halogen light source and an industrial norm material testing machine. Polarized light microscopy and hematoxylin-eosin staining indicated that lateral contraction of collagen structures is responsible for total intensity variations during a 20-cycle preconditioning and a 5-cycle tensile test. Tensile force progress is opposite to total transmission progress. Due to dehydration, wavelength-specific radiation intensity shifting is determined during the test, primarily noticeable in a water absorption band between 1400 and 1500 nm. The results show the capability of integrating spectrophotometry technology into biomechanics for determining structural alterations of human collagen due to applied strain. Being more sensitive to drying, spectrophotometry may likely serve as a quality control in stress-strain testing of biological structures.

Paper Details

Date Published: 24 June 2013
PDF: 4 pages
J. Biomed. Opt. 18(6) 060506 doi: 10.1117/1.JBO.18.6.060506
Published in: Journal of Biomedical Optics Volume 18, Issue 6
Show Author Affiliations
Markus Ernstberger, Westsächsische Hochschule Zwickau (Germany)
Freddy Sichting, Univ. Leipzig (Germany)
Tobias Baselt, Westsächsische Hochschule Zwickau (Germany)
Peter Hartmann, Westsächsische Hochschule Zwickau (Germany)
Gabriela Aust, Univ. Leipzig (Germany)
Niels Hammer, Univ. Leipzig (Germany)

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