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Journal of Biomedical Optics

Optical imaging for brain tissue characterization using relative fluorescence lifetime imaging
Author(s): Asael Papour; Zachary D. Taylor; Adria J. Sherman; Desiree Sanchez; Gregory Lucey; Linda Liau; Oscar M. Stafsudd; William H. Yong; Warren S. Grundfest
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Paper Abstract

An autofluorescence lifetime wide-field imaging system that can generate contrast in underlying tissue structures of normal and malignant brain tissue samples with video rate acquisition and processing time is presented. Images of the investigated tissues were acquired with high resolution (∼35  μm ) using an algorithm to produce contrast based on differences in relative lifetimes. Sufficient contrast for delineation was produced without the computation of fluorescence decay times or Laguerre coefficients. The imaged tissues were sent for histological analysis that confirmed the detected imaged tissues morphological findings and correlations between relative lifetime maps and histology identified.

Paper Details

Date Published: 12 June 2013
PDF: 4 pages
J. Biomed. Opt. 18(6) 060504 doi: 10.1117/1.JBO.18.6.060504
Published in: Journal of Biomedical Optics Volume 18, Issue 6
Show Author Affiliations
Asael Papour, Univ. of California, Los Angeles (United States)
Zachary D. Taylor, Univ. of California, Los Angeles (United States)
Adria J. Sherman, Univ. of California, Los Angeles (United States)
Desiree Sanchez, Univ. of California, Los Angeles (United States)
Gregory Lucey, Univ. of California, Los Angeles (United States)
Linda Liau, Univ. of California, Los Angeles (United States)
Oscar M. Stafsudd, Univ. of California, Los Angeles (United States)
William H. Yong, Univ. of California, Los Angeles (United States)
Warren S. Grundfest, Univ. of California, Los Angeles (United States)


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