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Journal of Biomedical Optics • Open Access

Fast optical sectioning obtained by structured illumination microscopy using a digital mirror device
Author(s): Dongli Xu; Tao Jiang; Anan Li; Bihe Hu; Zhao Feng; Hui Gong; Shaoqun Zeng; Qingming Luo

Paper Abstract

High-throughput optical imaging is critical to obtain large-scale neural connectivity information of brain in neuroscience. Using a digital mirror device and a scientific complementary metal-oxide semiconductor camera, we report a significant speed improvement of structured illumination microscopy (SIM), which produces a maximum SIM net frame rate of 133 Hz. We perform three-dimensional (3-D) imaging of mouse brain slices at diffraction-limited resolution and demonstrate the fast 3-D imaging capability to a large sample with an imaging rate of 6.9×10 7   pixel/s of our system, an order of magnitude faster than previously reported.

Paper Details

Date Published: 11 June 2013
PDF: 4 pages
J. Biomed. Opt. 18(6) 060503 doi: 10.1117/1.JBO.18.6.060503
Published in: Journal of Biomedical Optics Volume 18, Issue 6
Show Author Affiliations
Dongli Xu, Huazhong Univ. of Science and Technology (China)
Tao Jiang, Huazhong Univ. of Science and Technology (China)
Anan Li, Huazhong Univ. of Science and Technology (China)
Bihe Hu, Huazhong Univ. of Science and Technology (China)
Zhao Feng, Huazhong Univ. of Science and Technology (China)
Hui Gong, Huazhong Univ. of Science and Technology (China)
Shaoqun Zeng, Huazhong Univ. of Science and Technology (China)
Qingming Luo, Huazhong Univ. of Science and Technology (China)


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