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Journal of Biomedical Optics • Open Access

Laser guidance–based cell detection in a microfluidic biochip
Author(s): Wan Qin; Lucas Schmidt; Xiaoqi Yang; Lina Wei; Ting Huang; Julie X. Yuan; Xiang Peng; Xiaocong Yuan; Bruce Z. Gao

Paper Abstract

We developed a microfluidic biochip to perform laser guidance on two cell types, chick embryonic forebrain neurons and spinal cord neurons. Observation of neurons under a high-magnification microscope, which we obtained from these two cell types, showed no difference in morphology. However, when flowing in the microfluidic channel and simultaneously being laser guided, the two cell types gained quite different guidance speeds under the same experimental conditions. The results demonstrate that different cell types with the same morphology (e.g., size, shape, etc.) can be effectively distinguished from each other by measuring the difference in guidance speeds (the maximum flow speed minus the initial flow speed). This technique is expected to provide a new approach to high-throughput, label-free cell sorting with high sensitivity.

Paper Details

Date Published: 11 June 2013
PDF: 4 pages
J. Biomed. Opt. 18(6) 060502 doi: 10.1117/1.JBO.18.6.060502
Published in: Journal of Biomedical Optics Volume 18, Issue 6
Show Author Affiliations
Wan Qin, Clemson Univ. (United States)
Lucas Schmidt, Clemson Univ. (United States)
Xiaoqi Yang, Clemson Univ. (United States)
Lina Wei, Clemson Univ. (United States)
Ting Huang, Clemson Univ. (United States)
Julie X. Yuan, Clemson Univ. (United States)
Xiang Peng, Shenzhen Univ. (China)
Xiaocong Yuan, Nankai Univ. (China)
Bruce Z. Gao, Clemson Univ. (United States)

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