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Journal of Biomedical Optics • Open Access

Investigation of tryptophan–NADH interactions in live human cells using three-photon fluorescence lifetime imaging and Förster resonance energy transfer microscopyn1

Paper Abstract

A method to investigate the metabolic activity of intracellular tryptophan (TRP) and coenzyme-NADH using three-photon (3P) fluorescence lifetime imaging (FLIM) and Förster resonance energy transfer (FRET) is presented. Through systematic analysis of FLIM data from tumorigenic and nontumorigenic cells, a statistically significant decrease in the fluorescence lifetime of TRP was observed in response to the increase in protein-bound NADH as cells were treated with glucose. The results demonstrate the potential use of 3P-FLIM-FRET as a tool for label-free screening of the change in metabolic flux occurring in human diseases or other clinical conditions.

Paper Details

Date Published: 7 June 2013
PDF: 4 pages
J. Biomed. Opt. 18(6) 060501 doi: 10.1117/1.JBO.18.6.060501
Published in: Journal of Biomedical Optics Volume 18, Issue 6
Show Author Affiliations
Vinod Jyothikumar, Univ. of Virginia (United States)
Yuansheng Sun, Univ. of Virginia (United States)
Ammasi Periasamy, Univ. of Virginia (United States)


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