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Journal of Biomedical Optics

Label-free DNA biosensor based on a peptide nucleic acid-functionalized microstructured optical fiber-Bragg grating
Author(s): Alessandro Candiani; Alessandro Bertucci; Sara Giannetti; Maria Konstantaki; Alex Manicardi; Stavros Pissadakis; Annamaria Cucinotta; Roberto Corradini; Stefano Selleri
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Paper Abstract

We describe a novel sensing approach based on a functionalized microstructured optical fiber-Bragg grating for specific DNA target sequences detection. The inner surface of a microstructured fiber, where a Bragg grating was previously inscribed, has been functionalized by covalent linking of a peptide nucleic acid probe targeting a DNA sequence bearing a single point mutation implicated in cystic fibrosis (CF) disease. A solution of an oligonucleotide (ON) corresponding to a tract of the CF gene containing the mutated DNA has been infiltrated inside the fiber capillaries and allowed to hybridize to the fiber surface according to the Watson-Crick pairing. In order to achieve signal amplification, ON-functionalized gold nanoparticles were then infiltrated and used in a sandwich-like assay. Experimental measurements show a clear shift of the reflected high order mode of a Bragg grating for a 100 nM DNA solution, and fluorescence measurements have confirmed the successful hybridization. Several experiments have been carried out on the same fiber using the identical concentration, showing the same modulation trend, suggesting the possibility of the reuse of the sensor. Measurements have also been made using a 100 nM mismatched DNA solution, containing a single nucleotide mutation and corresponding to the wild-type gene, and the results demonstrate the high selectivity of the sensor.

Paper Details

Date Published: 10 May 2013
PDF: 7 pages
J. Biomed. Opt. 18(5) 057004 doi: 10.1117/1.JBO.18.5.057004
Published in: Journal of Biomedical Optics Volume 18, Issue 5
Show Author Affiliations
Alessandro Candiani, Univ. degli Studi di Parma (Italy)
Alessandro Bertucci, Univ. degli Studi di Parma (Italy)
Sara Giannetti, Univ. degli Studi di Parma (Italy)
Maria Konstantaki, Foundation for Research and Technology-Hellas (Greece)
Alex Manicardi, Univ. degli Studi di Parma (Italy)
Stavros Pissadakis, Foundation for Research and Technology-Hellas (Greece)
Annamaria Cucinotta, Univ. degli Studi di Parma (Italy)
Roberto Corradini, Univ. degli Studi di Parma (Italy)
Stefano Selleri, Univ. degli Studi di Parma (Italy)

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