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Journal of Biomedical Optics • Open Access

Confocal Raman data analysis enables identifying apoptosis of MCF-7 cells caused by anticancer drug paclitaxel
Author(s): Hamideh Salehi; Elodie Middendorp; Ivan Panayotov; Pierre-Yves Collard Dutilleul; Attila-Gergely Vegh; Sathish Kumar Ramakrishnan; Csilla Gergely; Frédéric J. G. Cuisinier

Paper Abstract

Confocal Raman microscopy is a noninvasive, label-free imaging technique used to study apoptosis of live MCF-7 cells. The images are based on Raman spectra of cells components, and their apoptosis is monitored through diffusion of cytochrome c in cytoplasm. K-mean clustering is used to identify mitochondria in cells, and correlation analysis provides the cytochrome c distribution inside the cells. Our results demonstrate that incubation of cells for 3 h with 10 μM of paclitaxel does not induce apoptosis in MCF-7 cells. On the contrary, incubation for 30 min at a higher concentration (100 μM) of paclitaxel induces gradual release of the cytochrome c into the cytoplasm, indicating cell apoptosis via a caspase independent pathway.

Paper Details

Date Published: 9 May 2013
PDF: 7 pages
J. Biomed. Opt. 18(5) 056010 doi: 10.1117/1.JBO.18.5.056010
Published in: Journal of Biomedical Optics Volume 18, Issue 5
Show Author Affiliations
Hamideh Salehi, Univ. Montpellier 1 (France)
Elodie Middendorp, Univ. Montpellier 1 (France)
Ivan Panayotov, Univ. Montpellier 1 (France)
Pierre-Yves Collard Dutilleul, Univ. Montpellier 1 (France)
Attila-Gergely Vegh, Univ. Montpellier 1 (France)
Sathish Kumar Ramakrishnan, Univ. Montpellier 2 (France)
Csilla Gergely, Univ. Montpellier 2 (France)
Frédéric J. G. Cuisinier, Univ. Montpellier 1 (France)

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