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Journal of Biomedical Optics

Single-walled carbon nanotubes as specific targeting and Raman spectroscopic agents for detection and discrimination of single human breast cancer cells
Author(s): Zeid A. Nima; Meena Mahmood; Alokita Karmakar; Thikra Mustafa; Shawn Bourdo; Yang Xu; Alexandru S. Biris
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Paper Abstract

Raman active nano-complex agents based on single-walled carbon nanotubes (SWCNTs) are prepared and used for the swift and specific detection of breast cancer cells. SWCNTs are functionalized to bond covalently with the anti-epithelial cell adhesion molecule (anti-EpCAM) antibody, which is specific to the highly expressed EpCAM antigen on the surface of breast cancer cells (MCF-7), but not on normal cells. The Raman nano-complexes demonstrate excellent ability to detect in vitro single breast cancer cells (MCF-7) and discriminate between them and normal fibroblast cells during the first 30 min of the targeting process. Raman linearity scanning is collected from a monolayer cell mixture, including both cancer cells and normal cells incubated with anti-EpCAM-SWCNTs, using a 633-nm laser excitation. The results shows that the Raman signal collected from targeted MCF-7 cells is extremely high, whereas there is little signal from the normal cells.

Paper Details

Date Published: 14 May 2013
PDF: 11 pages
J. Biomed. Opt. 18(5) 055003 doi: 10.1117/1.JBO.18.5.055003
Published in: Journal of Biomedical Optics Volume 18, Issue 5
Show Author Affiliations
Zeid A. Nima, Univ. of Arkansas at Little Rock (United States)
Meena Mahmood, Univ. of Arkansas at Little Rock (United States)
Alokita Karmakar, Univ. of Arkansas at Little Rock (United States)
Thikra Mustafa, Univ. of Arkansas at Little Rock (United States)
Shawn Bourdo, Univ. of Arkansas at Little Rock (United States)
Yang Xu, Univ. of Arkansas at Little Rock (United States)
Alexandru S. Biris, Univ. of Arkansas at Little Rock (United States)


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