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Journal of Biomedical Optics

Improved detectability of neuronal connectivity on mechanical sectioning setup by using confocal detection
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Paper Abstract

A recently reported micro-optical sectioning tomography system has great potential to draw the neuronal circuits of large brain volume with submicron resolution by combining fine mechanic sectioning with simultaneous optical imaging. However, sectioning the fluorescence sample sometimes induces tears between adjacent tiles and causes difficulties in continuous fiber tracing from fluorescence imaging. A confocal detection to recover the interruptions of the nerve fiber is introduced. With a 50-μm-width confocal slit, the signal-to-background ratio is increased 16- to 49-fold more than that without the slit, which effectively improves the detectability of the signal in the interruptions and enables continuous tracing of the neuronal circuits.

Paper Details

Date Published: 28 May 2013
PDF: 4 pages
J. Biomed. Opt. 18(5) 050506 doi: 10.1117/1.JBO.18.5.050506
Published in: Journal of Biomedical Optics Volume 18, Issue 5
Show Author Affiliations
Xiaoli Qi, Huazhong Univ. of Science and Technology (China)
Hanqing Xiong, Huazhong Univ. of Science and Technology (China)
Xiaohua Lv, Huazhong Univ. of Science and Technology (China)
Jianling Chen, Huazhong Univ. of Science and Technology (China)
Hui Gong, Huazhong Univ. of Science and Technology (China)
Qingming Luo, Huazhong Univ. of Science and Technology (China)
Shaoqun Zeng, Huazhong Univ. of Science and Technology (China)


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