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Journal of Biomedical Optics • Open Access

Noninvasive three-dimensional live imaging methodology for the spindles at meiosis and mitosis
Author(s): Jing-gao Zheng; Tiancheng Huo; Ning Tian; Tianyuan Chen; Chengming Wang; Ning Zhang; Fengying Zhao; Danyu Lu; Dieyan Chen; Wanyun Ma; Jia-Lin Sun; Ping Xue

Paper Abstract

The spindle plays a crucial role in normal chromosome alignment and segregation during meiosis and mitosis. Studying spindles in living cells noninvasively is of great value in assisted reproduction technology (ART). Here, we present a novel spindle imaging methodology, full-field optical coherence tomography (FF-OCT). Without any dye labeling and fixation, we demonstrate the first successful application of FF-OCT to noninvasive three-dimensional (3-D) live imaging of the meiotic spindles within the mouse living oocytes at metaphase II as well as the mitotic spindles in the living zygotes at metaphase and telophase. By post-processing of the 3-D dataset obtained with FF-OCT, the important morphological and spatial parameters of the spindles, such as short and long axes, spatial localization, and the angle of meiotic spindle deviation from the first polar body in the oocyte were precisely measured with the spatial resolution of 0.7 μm. Our results reveal the potential of FF-OCT as an imaging tool capable of noninvasive 3-D live morphological analysis for spindles, which might be useful to ART related procedures and many other spindle related studies.

Paper Details

Date Published: 9 May 2013
PDF: 4 pages
J. Biomed. Opt. 18(5) 050505 doi: 10.1117/1.JBO.18.5.050505
Published in: Journal of Biomedical Optics Volume 18, Issue 5
Show Author Affiliations
Jing-gao Zheng, Tsinghua Univ. (China)
Tiancheng Huo, Tsinghua Univ. (China)
Ning Tian, Tsinghua Univ. (China)
Tianyuan Chen, Tsinghua Univ. (China)
Chengming Wang, Tsinghua Univ. (China)
Ning Zhang, Tsinghua Univ. (United States)
Fengying Zhao, Tsinghua Univ. (China)
Danyu Lu, Peking Univ. (China)
Dieyan Chen, Tsinghua Univ. (China)
Wanyun Ma, Tsinghua Univ. (China)
Jia-Lin Sun, Tsinghua Univ. (China)
Ping Xue, Tsinghua Univ. (China)

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