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Journal of Biomedical Optics • Open Access

Fluorescence lifetime imaging and reflectance confocal microscopy for multiscale imaging of oral precancer
Author(s): Joey M. Jabbour; Shuna Cheng; Bilal H. Malik; Rodrigo Cuenca; Javier A. Jo; John Wright; Yi-Shing L. Cheng; Kristen C. Maitland

Paper Abstract

Optical imaging techniques using a variety of contrast mechanisms are under evaluation for early detection of epithelial precancer; however, tradeoffs in field of view (FOV) and resolution may limit their application. Therefore, we present a multiscale multimodal optical imaging system combining macroscopic biochemical imaging of fluorescence lifetime imaging (FLIM) with subcellular morphologic imaging of reflectance confocal microscopy (RCM). The FLIM module images a 16×16  mm 2 tissue area with 62.5 μm lateral and 320 ps temporal resolution to guide cellular imaging of suspicious regions. Subsequently, coregistered RCM images are acquired at 7 Hz with 400 μm diameter FOV, <1  μm lateral and 3.5 μm axial resolution. FLIM-RCM imaging was performed on a tissue phantom, normal porcine buccal mucosa, and a hamster cheek pouch model of oral carcinogenesis. While FLIM is sensitive to biochemical and macroscopic architectural changes in tissue, RCM provides images of cell nuclear morphology, all key indicators of precancer progression.

Paper Details

Date Published: 17 April 2013
PDF: 11 pages
J. Biomed. Opt. 18(4) 046012 doi: 10.1117/1.JBO.18.4.046012
Published in: Journal of Biomedical Optics Volume 18, Issue 4
Show Author Affiliations
Joey M. Jabbour, Texas A&M Univ. (United States)
Shuna Cheng, Texas A&M Univ. (United States)
Bilal H. Malik, Texas A&M Univ. (United States)
Rodrigo Cuenca, Texas A&M Univ. (United States)
Javier A. Jo, Texas A&M Univ. (United States)
John Wright, Texas A&M Health Science Ctr. (United States)
Yi-Shing L. Cheng, Texas A&M Health Science Ctr. (United States)
Kristen C. Maitland, Texas A&M Univ. (United States)


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