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Journal of Biomedical Optics • Open Access

Optical coherence tomography for assessment of microbicide safety in a small animal model
Author(s): Brent A. Bell; Kathleen L. Vincent; Nigel Bourne; Gracie Vargas; Massoud Motamedi

Paper Abstract

Sensitive imaging techniques for small animals are needed to assess drug toxicity in preclinical studies. Optical coherence tomography (OCT) provides a noninvasive tool for high-resolution, depth-resolved visualization of drug-induced changes in tissue morphology. In a mouse model, we utilize OCT to assess vaginal tissue integrity following the application of topical microbicides (drugs used to prevent infection). Mice are challenged with herpes simplex virus-2 (HSV-2) to determine the correlation of tissue damage as quantified by OCT to increased susceptibility. The microbicide benzalkonium chloride (BZK) (0.02, 0.2, or 2%) or phosphate buffered saline control is administered intravaginally. In vivo OCT imaging and collection of tissue samples are performed after treatment. A quantitative OCT scoring system is applied to assess epithelial damage, and the results are compared with those of histology. A separate group of mice are treated similarly then challenged with HSV-2. Epithelial morphology quantified noninvasively by OCT and histology are dose-dependent (p<0.0001 ). The OCT scoring system detected a significant increase in epithelial damage with increasing BZK concentration (p<0.0001 ). These results paralleled an increase in HSV-2 susceptibility (p<0.005 ). OCT can be used as a noninvasive tool to assess topical drug toxicity in a small animal model with potential to predict increased susceptibility to vaginal infection.

Paper Details

Date Published: 15 April 2013
PDF: 8 pages
J. Biomed. Opt. 18(4) 046010 doi: 10.1117/1.JBO.18.4.046010
Published in: Journal of Biomedical Optics Volume 18, Issue 4
Show Author Affiliations
Brent A. Bell, The Univ. of Texas Medical Branch (United States)
Kathleen L. Vincent, The Univ. of Texas Medical Branch (United States)
Nigel Bourne, The Univ. of Texas Medical Branch (United States)
Gracie Vargas, The Univ. of Texas Medical Branch (United States)
Massoud Motamedi, The Univ. of Texas Medical Branch (United States)

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